Cryo-EM structure of NKCC1 Fu_CTD
Resolution: 3.34 Å
EM Method: Single-particle
Fitted PDBs: 8ste
Q-score: 0.493
Moseng MA, Su CC, Rios K, Cui M, Lyu M, Glaza P, Klenotic PA, Delpire E, Yu EW
Sci Adv (2022) 8 pp. eabq0952- [ Pubmed: 36306358 DOI: doi:10.1126/sciadv.abq0952 ]
- Solute carrier family 12 member 2 (45 kDa, Protein from Homo sapiens)
- 5-(aminosulfonyl)-4-chloro-2-[(2-furylmethyl)amino]benzoic acid (330 Da, Ligand)
- Nkcc1_fu_ctd (Complex from Homo sapiens)
CryoEM structure of human NKCC1
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7mxo
Q-score: 0.42
Moseng MA, Su CC, Rios K, Cui M, Lyu M, Glaza P, Klenotic PA, Delpire E, Yu EW
Sci Adv (2022) 8 pp. eabq0952-eabq0952 [ Pubmed: 36306358 DOI: doi:10.1126/sciadv.abq0952 ]
- Solute carrier family 12 member 2 (102 kDa, Protein from human)
- Potassium ion (39 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- Full-length human nkcc1 with nacl and kcl (131 kDa, Complex from Homo sapiens)
CryoEM structure of human NKCC1 state Fu-I
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 7n3n
Q-score: 0.411
Moseng MA, Su CC, Rios K, Cui M, Lyu M, Glaza P, Klenotic PA, Delpire E, Yu EW
Sci Adv (2022) 8 pp. eabq0952-eabq0952 [ Pubmed: 36306358 DOI: doi:10.1126/sciadv.abq0952 ]
- Solute carrier family 12 member 2 (131 kDa, Protein from human)
- 5-(aminosulfonyl)-4-chloro-2-[(2-furylmethyl)amino]benzoic acid (330 Da, Ligand)
- Human nkcc1 in a detergent micelle with furosemide (131 MDa, Complex from Homo sapiens)
Resolution: 3.87 Å
EM Method: Single-particle
Fitted PDBs: 7sfl
Q-score: 0.374
CryoEM structure of NKCC1 Bu-I
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 7smp
Q-score: 0.47
Moseng MA, Su CC, Rios K, Cui M, Lyu M, Glaza P, Klenotic PA, Delpire E, Yu EW
Sci Adv (2022) 8 pp. eabq0952-eabq0952 [ Pubmed: 36306358 DOI: doi:10.1126/sciadv.abq0952 ]
- 3-(butylamino)-4-phenoxy-5-sulfamoylbenzoic acid (364 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Solute carrier family 12 member 2 (103 kDa, Protein from Homo sapiens)
- Nkcc1 bu-i (Cellular component from Homo sapiens)
Cryo-electron microscopy structure of the heavy metal efflux pump CusA in the symmetric closed state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7kf5
Q-score: 0.424
Moseng MA, Lyu M, Pipatpolkai T, Glaza P, Emerson CC, Stewart PL, Stansfeld PJ, Yu EW
Mbio (2021) 12 [ DOI: doi:10.1128/mBio.00452-21 Pubmed: 33820823 ]
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7kf6
Q-score: 0.391
Moseng MA, Lyu M, Pipatpolkai T, Glaza P, Emerson CC, Stewart PL, Stansfeld PJ, Yu EW
Mbio (2021) 12 [ DOI: doi:10.1128/mBio.00452-21 Pubmed: 33820823 ]
- Copper (i) ion (63 Da, Ligand)
- Cation efflux system protein cusa (115 kDa, Protein from Escherichia coli)
- Cusa in symmetric copper binding form (Complex from Escherichia coli)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7kf7
Q-score: 0.519
Moseng MA, Lyu M, Pipatpolkai T, Glaza P, Emerson CC, Stewart PL, Stansfeld PJ, Yu EW
Mbio (2021) 12 [ DOI: doi:10.1128/mBio.00452-21 Pubmed: 33820823 ]
- Copper (i) ion (63 Da, Ligand)
- Cation efflux system protein cusa (115 kDa, Protein from Escherichia coli)
- Cusa with heterogeneous protomer conformations (Complex from Escherichia coli)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7kf8
Q-score: 0.492
Moseng MA, Lyu M, Pipatpolkai T, Glaza P, Emerson CC, Stewart PL, Stansfeld PJ, Yu EW
Mbio (2021) 12 [ DOI: doi:10.1128/mBio.00452-21 Pubmed: 33820823 ]
- Copper (i) ion (63 Da, Ligand)
- Cation efflux system protein cusa (115 kDa, Protein from Escherichia coli)
- Cusa with heterogeneous protomer conformations (Complex from Escherichia coli)
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 6vks
Q-score: 0.48
Lyu M, Moseng MA, Reimche JL, Holley CL, Dhulipala V, Su CC, Shafer WM, Yu EW
Mbio (2020) 11 [ DOI: doi:10.1128/mBio.00996-20 Pubmed: 32457251 ]
- Phosphatidylethanolamine (734 Da, Ligand)
- Efflux pump membrane transporter (112 kDa, Protein from Neisseria gonorrhoeae)
- Mtrd efflux pump (300 kDa, Complex from Neisseria gonorrhoeae)
- (2r,4s)-2-[(1r)-1-{[(2r)-2-amino-2-phenylacetyl]amino}-2-oxoethyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid (351 Da, Ligand)